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pet28 expression vector  (Millipore)


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    Structured Review

    Millipore pet28 expression vector
    Pet28 Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28+expression+vector/pet28a/pmc11070930-722-8-11
    Average 90 stars, based on 1 article reviews
    pet28 expression vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Construct:

    Article Title: Novel modifications of PARP inhibitor veliparib increase PARP1 binding to DNA breaks
    Article Snippet: The IC 50 values for target compounds against PARP1 enzyme were calculated from a 10-point concentration-response curve using the BPS PARP1 Chemiluminescence Activity Assay Kit (Catalog #80551) (BPS Bioscience). .. The following PARP1 constructs were expressed from a pET28 expression vector (Novagen) with an N-terminal hexahistidine tag: PARP1 WT (residues 1–1014), ART domain (residues 661–1011 Δ678–787) and Zn1-Zn3 ΔZn2 domains (residues 1–366 Δ97–206). .. The WGR-CAT domain (518–1014) with the ΔVE mutation was expressed from a pET24 expression vector (Novagen) with a C-terminal hexahistidine tag.

    Article Title: Modification of Superoxide Dismutase 1 (SOD1) Properties by a GFP Tag – Implications for Research into Amyotrophic Lateral Sclerosis (ALS)
    Article Snippet: GFP-tagging was achieved by subcloning SOD1 cDNAs into the vector pAcGFP1-C1 (Clontech) between the EcoRI and SalI restriction sites. .. Constructs were cloned into the pET28 expression vector (Novagen), downstream of the T7 promoter between NdeI and XhoI sites introducing a thrombin cleavage site between the protein and the N-terminal His-tag. .. All constructs were transformed into Escherichia coli strain BL21 (DE3) (Novagen) according to manufacturer's protocol.

    Article Title: Blomia tropicalis –Specific TCR Transgenic Th2 Cells Induce Inducible BALT and Severe Asthma in Mice by an IL-4/IL-13–Dependent Mechanism
    Article Snippet: .. The construct was designed and codon optimized for expression in the E. coli bacteria by the OptimumGene algorithm (GenScript, Piscataway, NJ) and cloned into a pET28 expression vector (EMD Millipore, Billerica, MA). ..

    Expressing:

    Article Title: Novel modifications of PARP inhibitor veliparib increase PARP1 binding to DNA breaks
    Article Snippet: The IC 50 values for target compounds against PARP1 enzyme were calculated from a 10-point concentration-response curve using the BPS PARP1 Chemiluminescence Activity Assay Kit (Catalog #80551) (BPS Bioscience). .. The following PARP1 constructs were expressed from a pET28 expression vector (Novagen) with an N-terminal hexahistidine tag: PARP1 WT (residues 1–1014), ART domain (residues 661–1011 Δ678–787) and Zn1-Zn3 ΔZn2 domains (residues 1–366 Δ97–206). .. The WGR-CAT domain (518–1014) with the ΔVE mutation was expressed from a pET24 expression vector (Novagen) with a C-terminal hexahistidine tag.

    Article Title: Essential roles of buried phenylalanine in the structural stability of thioredoxin from a psychrophilic Arctic bacterium Sphingomonas sp.
    Article Snippet: .. The pET28 expression vector was purchased from Novagen (Madison, WI, USA). .. The TA vector, restriction enzymes, and pfu polymerase were acquired from Enzynomics (Daejeon, South Korea).

    Article Title: Non-protective immune imprint underlies failure of Staphylococcus aureus IsdB vaccine.
    Article Snippet: .. The PCR product was cloned into pET28 expression vector (Novagen) and expressed as described previously with somemodifications (1). .. Briefly, IsdB-expressing plasmids were used to transform E. coli BL21 (DE3) cells (NEB) to produce a His-tagged protein with 1mM of isopropyl-b-D-thiogalactoside (IPTG) for 2 hours.

    Article Title: Modification of Superoxide Dismutase 1 (SOD1) Properties by a GFP Tag – Implications for Research into Amyotrophic Lateral Sclerosis (ALS)
    Article Snippet: GFP-tagging was achieved by subcloning SOD1 cDNAs into the vector pAcGFP1-C1 (Clontech) between the EcoRI and SalI restriction sites. .. Constructs were cloned into the pET28 expression vector (Novagen), downstream of the T7 promoter between NdeI and XhoI sites introducing a thrombin cleavage site between the protein and the N-terminal His-tag. .. All constructs were transformed into Escherichia coli strain BL21 (DE3) (Novagen) according to manufacturer's protocol.

    Article Title: Blomia tropicalis –Specific TCR Transgenic Th2 Cells Induce Inducible BALT and Severe Asthma in Mice by an IL-4/IL-13–Dependent Mechanism
    Article Snippet: .. The construct was designed and codon optimized for expression in the E. coli bacteria by the OptimumGene algorithm (GenScript, Piscataway, NJ) and cloned into a pET28 expression vector (EMD Millipore, Billerica, MA). ..

    Article Title: Conformational Disorder Analysis of the Conditionally Disordered Protein CP12 from Arabidopsis thaliana in Its Different Redox States
    Article Snippet: .. The cDNA coding for the mature form of the isoform 2 of Arabidopsis thaliana CP12 (gene ID At3g62410) was cloned into the pET28 expression vector (Novagen, Madison, WI, USA) in frame with an N-terminal 6XHis tag, followed by a thrombin cleavage site. ..

    Article Title: Duplication and Diversification of the Spermidine/Spermine N 1 -acetyltransferase 1 Genes in Zebrafish
    Article Snippet: Enzymes used in molecular cloning were obtained from New England Biolabs. .. The pGEX-2T expression vector was from GE Healthcare Bioscience; the pcDNA3.1/myc-His vector was from Invitrogen; the pET28 expression vector and the Escherichia coli BL21 (DE3) host cells were from Novagen. .. In order to investigate the presence of SSAT1-related genes in invertebrate and vertebrate deuterostomes, human SSAT1 (NM_002970) and SSAT2 (NM_133491) were used as templates for TBlastN searches at the National Center for Biotechnology Information (NCBI) website ( http://www.ncbi.nlm.nih.gov/mapview/ ) for sea urchin ( Strongylocentrotus purpuratus ), sea squirt ( Ciona intestinalis ), zebrafish ( Danio rerio ), and mouse ( Mus musculus ) genomes, or in Ensembl ( http://www.ensembl.org/index.html ) for medaka ( Oryzias latipes ), stickleback ( Gasterosteus aculeatus ), Takifugu ( Takifugu rubripes ), and Tetraodon ( Tetraodon nigroviridis ) genomes.

    Plasmid Preparation:

    Article Title: Novel modifications of PARP inhibitor veliparib increase PARP1 binding to DNA breaks
    Article Snippet: The IC 50 values for target compounds against PARP1 enzyme were calculated from a 10-point concentration-response curve using the BPS PARP1 Chemiluminescence Activity Assay Kit (Catalog #80551) (BPS Bioscience). .. The following PARP1 constructs were expressed from a pET28 expression vector (Novagen) with an N-terminal hexahistidine tag: PARP1 WT (residues 1–1014), ART domain (residues 661–1011 Δ678–787) and Zn1-Zn3 ΔZn2 domains (residues 1–366 Δ97–206). .. The WGR-CAT domain (518–1014) with the ΔVE mutation was expressed from a pET24 expression vector (Novagen) with a C-terminal hexahistidine tag.

    Article Title: Non-protective immune imprint underlies failure of Staphylococcus aureus IsdB vaccine.
    Article Snippet: .. The PCR product was cloned into pET28 expression vector (Novagen) and expressed as described previously with somemodifications (1). .. Briefly, IsdB-expressing plasmids were used to transform E. coli BL21 (DE3) cells (NEB) to produce a His-tagged protein with 1mM of isopropyl-b-D-thiogalactoside (IPTG) for 2 hours.

    Article Title: Modification of Superoxide Dismutase 1 (SOD1) Properties by a GFP Tag – Implications for Research into Amyotrophic Lateral Sclerosis (ALS)
    Article Snippet: GFP-tagging was achieved by subcloning SOD1 cDNAs into the vector pAcGFP1-C1 (Clontech) between the EcoRI and SalI restriction sites. .. Constructs were cloned into the pET28 expression vector (Novagen), downstream of the T7 promoter between NdeI and XhoI sites introducing a thrombin cleavage site between the protein and the N-terminal His-tag. .. All constructs were transformed into Escherichia coli strain BL21 (DE3) (Novagen) according to manufacturer's protocol.

    Article Title: Blomia tropicalis –Specific TCR Transgenic Th2 Cells Induce Inducible BALT and Severe Asthma in Mice by an IL-4/IL-13–Dependent Mechanism
    Article Snippet: .. The construct was designed and codon optimized for expression in the E. coli bacteria by the OptimumGene algorithm (GenScript, Piscataway, NJ) and cloned into a pET28 expression vector (EMD Millipore, Billerica, MA). ..

    other:

    Article Title: Structural insights into kinetoplastid coronin oligomerization domain and F-actin interaction
    Article Snippet: The T. brucei Coronin coiled-coil domain was amplified using forward and reverse primers GAATTC TCGCAGTTGTTAGCTCTTGCCTCG and CTCGAG GGCAAGGGCCTTTATCTTTG CGAT containing BamHI and EcoRI restriction sites (Bold) designed manually from T.brucei strain (927/4 GUTat10.1) and L.donovani strain (BPK282A1) and the PCR product ligated with T/A vector pTZ57 R/T (Ins TA cloneTM PCR cloning kit, Fermentas International Inc.) TA clone digested and sub clone into pET28 (a) (Novagen) expression vector.

    Polymerase Chain Reaction:

    Article Title: Non-protective immune imprint underlies failure of Staphylococcus aureus IsdB vaccine.
    Article Snippet: .. The PCR product was cloned into pET28 expression vector (Novagen) and expressed as described previously with somemodifications (1). .. Briefly, IsdB-expressing plasmids were used to transform E. coli BL21 (DE3) cells (NEB) to produce a His-tagged protein with 1mM of isopropyl-b-D-thiogalactoside (IPTG) for 2 hours.

    Clone Assay:

    Article Title: Non-protective immune imprint underlies failure of Staphylococcus aureus IsdB vaccine.
    Article Snippet: .. The PCR product was cloned into pET28 expression vector (Novagen) and expressed as described previously with somemodifications (1). .. Briefly, IsdB-expressing plasmids were used to transform E. coli BL21 (DE3) cells (NEB) to produce a His-tagged protein with 1mM of isopropyl-b-D-thiogalactoside (IPTG) for 2 hours.

    Article Title: Modification of Superoxide Dismutase 1 (SOD1) Properties by a GFP Tag – Implications for Research into Amyotrophic Lateral Sclerosis (ALS)
    Article Snippet: GFP-tagging was achieved by subcloning SOD1 cDNAs into the vector pAcGFP1-C1 (Clontech) between the EcoRI and SalI restriction sites. .. Constructs were cloned into the pET28 expression vector (Novagen), downstream of the T7 promoter between NdeI and XhoI sites introducing a thrombin cleavage site between the protein and the N-terminal His-tag. .. All constructs were transformed into Escherichia coli strain BL21 (DE3) (Novagen) according to manufacturer's protocol.

    Article Title: Blomia tropicalis –Specific TCR Transgenic Th2 Cells Induce Inducible BALT and Severe Asthma in Mice by an IL-4/IL-13–Dependent Mechanism
    Article Snippet: .. The construct was designed and codon optimized for expression in the E. coli bacteria by the OptimumGene algorithm (GenScript, Piscataway, NJ) and cloned into a pET28 expression vector (EMD Millipore, Billerica, MA). ..

    Article Title: Conformational Disorder Analysis of the Conditionally Disordered Protein CP12 from Arabidopsis thaliana in Its Different Redox States
    Article Snippet: .. The cDNA coding for the mature form of the isoform 2 of Arabidopsis thaliana CP12 (gene ID At3g62410) was cloned into the pET28 expression vector (Novagen, Madison, WI, USA) in frame with an N-terminal 6XHis tag, followed by a thrombin cleavage site. ..

    Bacteria:

    Article Title: Blomia tropicalis –Specific TCR Transgenic Th2 Cells Induce Inducible BALT and Severe Asthma in Mice by an IL-4/IL-13–Dependent Mechanism
    Article Snippet: .. The construct was designed and codon optimized for expression in the E. coli bacteria by the OptimumGene algorithm (GenScript, Piscataway, NJ) and cloned into a pET28 expression vector (EMD Millipore, Billerica, MA). ..



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